Michael Dyson
Molecular Biologist and protein biochemist working with in Biochemistry Department, University of Cambridge with John McCafferty developing new protein interaction and recombinant antibody technologies.
| Headline: | Scientist |
| Industries: | Biotech, Nanotech |
| Skills: | Business, Editing, Management, Public speaking, Recruitment, Writing |
| Location: | University of Cambridge |
| Groups: | CUTEC |
| Interested in: | Consulting opportunities, Offering Expertise, Professional opportunities |
| Schools: | University of Birmingham |
WORK EXPERIENCE
| Employer: | Department of Biochemistry, University of Cambridge, England |
| Position: | Senior Research Associate |
| Time period: | September 2007 - Present |
| Description: | Research has focused on the biological validation of orphan receptor ligands by the generation of phage display selected antibodies, followed by antibody expression in E. coli or mammalian cells, and the use of antibodies in immunohistochemistry with mouse frozen embryos and mass spectrometry. New methods for high level monoclonal IgG expression are being optimized by transient transfection of mammalian cells. |
| Employer: | The Wellcome Trust Sanger Institute, Cambridge, England |
| Position: | Project Leader – Protein Expression |
| Time period: | May 2002 - August 2007 |
| Employer: | Sense Proteomic Ltd. (Procognia), Cambridge, England |
| Position: | Group Leader – Research Projects |
| Time period: | December 2000 - April 2002 |
| Employer: | Acambis PLC (Peptide Therapeutics PLC), Cambridge, England |
| Position: | Head of the Protein Engineering Section |
| Time period: | January 1997 - December 2000 |
| Employer: | Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh, Scotland |
| Position: | Post-doctoral Research Fellow with Professor Sir Kenneth Murray, Biogen Professor of Molecular Biology |
| Time period: | January 1992 - December 1997 |
| Employer: | Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA |
| Position: | Post-doctoral Research Associate (and Visiting Scientist in 1994) with Professor U. L. RajBhandary |
| Time period: | January 1989 - December 1992 |
EDUCATION
| University: | University of Birmingham |
| Time period: | 1989 |
| Degree: | Organic Chemistry , PhD |
| University: | University of Birmingham |
| Time period: | 1986 |
| Degree: | Chemistry, BSc |
PUBLICATIONS
| Articles: | Dyson, M. R., Global expression and identification of novel orphan IgSF receptor interactions, in preparation.
Dyson M. R., Perera R. L., Shadbolt S. P., Biderman L., Bromek K., Murzina N. V and McCafferty J. (2008) Identification of soluble protein fragments by gene fragmentation and genetic selection. Nucleic Acids Res. May;36(9):e51. Schofield D. J., Pope A. R., Clementel V., Buckell J., Chapple S. D., Clarke K. F., Conquer J. S., Crofts A. M., Crowther S. R., Dyson M. R. et al (2007) Application of phage display to high throughput antibody generation and characterization. Genome Biol. 8(11):R254. “Highly accessed” (Times cited = 1) Chapple, S., Crofts, A., Shadbolt, S. P., McCafferty, J. and Dyson, M. R. (2006) Multiplexed expression and screening for recombinant protein production in mammalian cells. BMC Biotechnol., 6: 49. “Highly accessed” (Times cited = 2) Dyson M. R., Shadbolt S. P., Vincent K. J., Perera R. L., McCafferty J. (2004) Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression. BMC Biotechnol., 4:32. (Times cited = 37) “Highly accessed” Tan, W. S., Dyson, M. R. and Murray, K. (2003) Hepatitis B virus core antigen: enhancement of its production in Escherichia coli, and interaction of the core particles with the viral surface antigen. Biol. Chem. 384, 363-371. (Times cited = 13) Tan, W. S., Dyson, M. R. and Murray, K. (1999) Two distinct segments of the hepatitis B virus surface antigen contribute synergistically to its association with the viral core particles. J. Mol. Biol., 286, 797-808. (Times cited = 22) Ramesh, V., Mayer, C., Dyson, M. R., Gite, S. and RajBhandary, U. L. (1999) Induced fit of a peptide loop of methionyl-tRNA formyltransferase triggered by the initiator tRNA substrate. Proc. Natl. Acad. Sci. USA, 96, 875-880. (Times cited = 11) Bottcher, B., Dyson, M. R., and Crowther, R. A. (1998). Finding the small difference: a nine amino acid extension to the hepatitis B core protein. Proc. Intl. Congr. Elect. Microsc., 14, 737-738. Bottcher, B., Tsuji, N., Takahashi, H., Dyson, M. R., Zhao, S., Crowther, R. A. and Murray, K. (1998) Peptides that block hepatitis B virus assembly: analysis by cryomicroscopy, mutagenesis and transfection. EMBO J., 17, 6839-6845. (Times cited = 39) Tsuji, N., Dyson, M. R., Cuervo, J. H., Abedi, J. K., Murray, K. and Takahashi, H. (1996) Inhibition of hepatitis B virus production in transfected hepatoma cells. Hepatology, 24 (4), Pt2 SS, 385. Dyson, M. R., Zhao, S. and Murray, K. (1996) Selection of ligands for hepatitis B core antigen. Prog. Biophys. Mol. Biol., 65, S1. Dyson, M. R. and Murray, K. (1995) Selection of peptide inhibitors of interactions involved in complex protein assemblies: Association of the core and surface antigens hepatitis B virus. Proc. Natl. Acad. Sci. USA 92, 2194-2198. (Times cited = 59) Dyson, M. R., Germaschewski, V. and Murray, K. (1995) Direct measurement via phage titre of the dissociation constants in solution of fusion phage-substrate complexes. Nucleic Acids Res., 23, 1531-1535. (Times cited = 13) Dyson, M. R., Mandal, N. and RajBhandary, U. L. (1993) Relationship between the structure and function of Escherichia coli initiator transfer-RNA. Biochimie 75, 1051-1060. (Times cited = 5) Lee, C. P., Mandal, N., Dyson, M. R. and RajBhandary, U.L. (1993) The discriminator base influences transfer RNA structure at the end of the acceptor stem and possibly its interaction with proteins. Proc. Natl. Acad. Sci. USA 90, 7149-7152. (Times cited = 39) Lee, C. P., Dyson, M. R., Mandal, N., Varshney, U., Bahramian, B. and RajBhandary, U. L. (1992) Striking effects of coupling mutations in the acceptor stem on recognition of tRNAs by Escherichia coli Met-tRNA synthetase and Met-tRNA transformylase. Proc. Natl. Acad. Sci. USA 89, 9262-9266. (Times cited = 61) Koole, L. H., Plavec, J., Liu, H. Y., Vincent, B. R., Dyson, M. R., Coe, P. L., Walker, R. T., Hardy, G. W., Rahim, S. G. and Chattopadhyaya, J. (1992) Conformation of two 2’-deoxy-4’-thionucleoside analogues studied by 500 MHz 1H NMR spectroscopy and X-ray crystallography. J. Amer. Chem. Soc., 114, 9936-9943. (Times cited = 51) Dyson, M. R., Coe, P. L. and Walker, R. T. (1991) The synthesis and antiviral activity of some 4’-thio-2’-deoxy-nucleoside analogues. J. Med. Chem., 34, 2782-2786. (Times cited = 131) Dyson, M. R., Coe, P. L. and Walker, R. T. (1991) An improved synthesis of benzyl 3,5-di-O-benzyl-2-deoxy-1,4-dithio-D-erythro-pentofuranoside, an intermediate in the synthesis of 4’-thionucleosides. Carbohydrate Research, 216, 237-248. (Times cited = 62) Dyson, M. R., Coe, P. L. and Walker, R. T. (1991) The synthesis and antiviral activity of some 4’-thio-2’-deoxy-nucleoside analogues. Nucleic Acids Res. Symp. Ser., 24, 1-4. Dyson, M. R., Coe, P. L. and Walker, R. T. (1991) The synthesis and antiviral properties of E-5-(2-bromovinyl)-4’-thio-2’-deoxyuridine. J. Chem. Soc., Chem Comm., 741-741. (Times cited = 49) |
| Books: | Dyson M.R. (2007) Expression strategy in Expression Systems: Methods Express, Eds Dyson M.R. and Durocher Y. (Scion Publishing Ltd., Oxford), 2007.
Dyson, M. R., Lee, C. P., Mandal, N., Seong, B. L., Varshney, U. and RajBhandary, U. L. (1993) Identity of a prokaryotic initiator tRNA in The Translational Apparatus, ed Nierhaus, K. H. (Plenum Press, New York), 23-33. |
| Patents: | Dyson M. R. et al, PCT Application No. PCT/IB03/05258 filed on September 16 2003. Arrays and Methods for Analysis of Drug Metabolising Enzymes.
Dyson, M. R. Hart D. J., Samaddar, M., Kozlowski, R. Z. and Blackburn, J. M., US Patent Application 20060199220, Protein Arrays and uses thereof. Dyson, M. R. et al , US Patent Application 20050221308, Protein tag comprising a biotinylation domain and method for increasing solubility and determining folding state. Dyson, M. R. et al., US Patent Application 20050214285. Epitopes or mimotopes derived from the C-epsilon-3 or C-epsilon-4 domains of IgE, antagonists thereof, and their therapeutic uses. Dyson, M. R. and Murray, K., US Patent 6,544520. Novel Hepatitis B inhibitors. My PhD work resulted in US Patent 5,356.882. |
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Wallis Motta
I would like to meet entrepreneurs in Cambridge, UK in order to collaborate with them or interview them on their experiences of starting a business, as part of my PhD research project... Are you interested? Send me a message!